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Associates Degree in General Science With a Technical Competence Area (TCA) in Biotechnology
TCA Courses
- Introduction To Biotechnology teaches:
- Pipetting and dilution skills
- Use and care of basic lab instruments - balances, pH meters, conductivity meters, spectrophotometers (vis and UV), centrifuges, gel electrophoresis chambers, thermal cyclers, autoclaves, incubators, laminar flow hoods
- Solution and buffer preparation
- How to keep a lab notebook and importance of documentation
- How to write an SOP
- Quality control, quality assurance, industry regulations and standards
- Cell Culture teaches how to:
- Use aseptic technique in culturing cells
- Establish primary cultures
- Maintain, count, split, and freeze cell lines
- Check cultures for contamination, including mycoplasma
- Determine cell viability
- Keep lab notebook
- Maintain CO 2 incubators and laminar flow hoods
The course's didactic instruction explains the biology and special needs of cultured cells and explores stem cells, cancer cells, and transformed cells.
- Bioinstrumentation teaches students to produce a biological product by:
- Examining the function of an enzyme (a-amylase) in Bacillus
- Isolating the gene for a-amylase and introducing the gene into E. coli using screening methods, gel electrophoresis, and PCR
- Isolating and purifying large quantities of a-amylase using chromatography and spectrophotometry
- Using probes to prove the identify of the product
- Examine yield and purity
- Keeping a lab notebook
- Internship - students work a minimum of 240 hours with a mentor. The last week of the semester is spent in class, presenting experiences with fellow students.
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